Abstract: In order to understand the origin and development of pteridophytes in Dinghushan National Nature Reserve, the wild pteridophyte resources in the reserve were comprehensively investigated from 2019 to 2020, and the species characteristics and distribution types of pteridophytes in this area were analyzed. The results showed that: (1) The pteridophytes in Dinghushan National Nature Reserve were composed of 123 species belonging to 62 genera and 29 families. (2) The families were mainly composed of Oligopyridae and Monopodidae, and the genus was mainly composed of single species. (3) From the ecological type perspective, native ferns were the main species in this area, accounting for 61.78% of the total pteridophytes. (4) The pteridophyte flora had obvious tropical characteristics at the level of family, genus and species, and at the level of species, tropical Asia accounted for the largest proportion, including three endemic species in China; (5) Dinghushan Nature Reserve had the closest relationship with Xiangshan Nature Reserve.