火草叶片愈伤组织诱导及其分化研究

Study on Callus Induction and Differentiation of Gerbera delavayi Leaves

摘要: 以火草叶片为外植体，研究了愈伤组织诱导与分化的方法。结果表明，火草无菌叶片在WPM+0.6 mg·L⁻¹6-BA+0.45 mg·L⁻¹NAA+30 g·L⁻¹蔗糖的培养基中，能诱导出直径0.5 cm、结构致密、质地硬的绿色愈伤组织，愈伤组织在WPM+0.75 mg·L⁻¹6-BA+0.15 mg·L⁻¹NAA+30 g·L⁻¹蔗糖的培养基中培养30 d分化出芽，平均每个分化的愈伤组织长出5.78个芽，芽苗转入1/2DKW+1.0 mg·L⁻¹ IBA +0.1mg·L⁻¹ NAA+30 g·L⁻¹蔗糖的培养基中培养30 d后，发育出5—13条不定根，且带有根毛的发达根系。

Abstract: Using Gerbera delavayi leaves as explants, the methods of callus induction and differentiation were studied. The results showed that the green callus with a diameter 0.5 cm, compact structure and hard texture could be induced from the sterile leaves of Gerbera delavayi in the medium of WPM+ 0.6 mg·L⁻¹6-BA + 0.45 mg·L⁻¹ NAA + 30 g·L⁻¹ sucrose. The calli differentiated into adventitious buds after 30 days in the medium of WPM+ 0.75 mg·L⁻¹6-BA+ 0.15 mg·L⁻¹ NAA+30 g·L⁻¹ sucrose. After the shoots were transferred to 1/2DKW+1.0 mg·L⁻¹IBA+0.1 mg·L⁻¹ NAA+30 g·L⁻¹ sucrose for 30 days, 5-13 adventitious roots with root hairs were developed.