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轻木萌发条件、低温耐受性及最适pH值条件探索

Exploration on Seed Germination Conditions, Low Temperature Tolerance and optimum pH value for Ochroma lagopus Growth

  • 摘要: 轻木(Ochroma lagopus)是木棉科多年生木本植物,其木材主要用于制造风力发电机叶片;然而我国轻木完全依赖进口。为保障新能源产业稳定发展,轻木国产化刻不容缓;本研究探索轻木种子的最佳萌发条件、轻木对低温的耐受性以及苗木生长的最适pH值,完善轻木种植技术,指导轻木国产化种植。 (1)轻木种子浸泡过夜后分别置于45℃、50℃、55℃、60℃、65℃、70℃、75℃、80℃水浴中处理50 min后,置于培养基上萌发;(2)轻木幼苗分别在0℃、2℃、4℃、6℃、8℃低温条件下处理3 d后,比较损伤率和恢复指数;(3)轻木植株用不同pH值的水持续浇灌调节土壤pH值,设置4.0、4.5、5.0、5.5、6.0、6.5、7.0七个pH值进行持续处理,并统计轻木幼苗120 d的生长量。轻木种子55℃处理后萌发率最高,可达80% ;而未处理的种子萌发率仅34%,热处理提高了轻木种子的萌发率。低温处理结果显示4℃、6℃、8℃处理幼苗几乎不受损伤,0℃和2℃幼苗叶片损伤严重;0℃、2℃和4℃处理后幼苗死亡率分别为79.24%、35.83%和6.28%。5℃和4℃低温处理土培苗3 d后死亡率分别为58.33%和33.33%。不同pH值条件下轻木长势统计结果显示pH 值6.0时轻木长势最佳,平均株高增长量为188.67 cm,而pH值 4.0时,株高平均增长量仅102.26 cm,pH 值6.0比pH 值4.0增长量高84.97%。研究表明轻木种子最佳萌发条件为种子浸泡后55℃热处理50 min,4℃以上低温对轻木损伤不明显,轻木种植最适pH值为6.0。

     

    Abstract: Ochroma lagopus is a perennial woody plant of the kapok family which also named balsa wood, and its wood is mainly used to make blades of wind driven generator. However, the balsa wood needed in China is completely imported from Indonesia and Ecuador, thus the localization of balsa wood is the guarantee to ensure the sustainable development of Chinese wind power industry. This research aimed to investigate the optimal seed germination conditions, the suitable pH for plants growth, and the cold tolerance of Ochroma lagopus under low temperature. This study improved the planting technology and lay the foundation for the localization of Ochroma lagopus industry. The seeds before germination were constantly treated 50 min at 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, 75℃ and 80℃ in the water bath. Two-month-old seedlings were cultured at 0℃, 2℃, 4℃, 6℃ and 8℃for 3 days to discover the low temperature tolerance limits of balsa wood. The seedlings of Ochroma lagopus were planted in pots with pH values of 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, and 7.0. The increment height of each plant was recorded weekly for 120 days. The germination rate of seeds was the highest after treatment at 55℃, which could reach 80%. However, the germination rate of control seeds was only 34%, and heat treatment obviously improved the germination rate of light wood seeds. The aseptic seedling exhibited low death rate under 4-8℃ conditions; while the seedlings exhibited 79.24%, 35.83% and 6.28% death rate under 0℃, 2℃ and 4℃ conditions, respectively. The seedlings grown in the soil were also treated with 8℃, 6℃, 5℃ and 4℃ for 3 days, the death rate of seedlings was 33.33% and 58.33% when treated with 5℃ and 4℃, respectively. The results showed that the growth was the best at pH 6.0, with an average plant height increase of 188.67 cm, while the height was only 102.26 cm at pH 4.0. During the 120 days, the height of plants grown at pH 6.0 were 84.97% taller than that of pH 4.0 condition. According to these results, the optimal balsa wood seed germination condition was that the seeds were soaked at 55℃ for 50 min, and the low temperature above 4℃ had no obvious damage to Ochroma lagopus; and the optimum pH value for planting Ochroma lagopus was 6.0.

     

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