Abstract: It is of great significance to explore the land use conflict in Chengdu-Chongqing urban agglomeration to promote regional sustainable development. The characteristics of land use change in Chengdu-Chongqing urban agglomeration from 2010 to 2020 were analyzed, and the land use conflict were calculated by landscape ecological risk assessment method, and its differentiation characteristics were studied by spatial autocorrelation and geostatistics methods. The results showed that: (1) The land use of Chengdu-Chongqing urban agglomeration was mainly cultivated land and forest land, and the area of land use types showed a trend of "three increases and three decreases", and the area of construction land and forest land increased obviously. (2) The overall level of land use conflicts in Chengdu-Chongqing urban agglomeration were mainly of controllable, but the intensity of conflicts had increased, and the spatial distribution had obvious spatial agglomeration. The intensity of conflicts varied with different land types, and the conflicts mainly focused on cultivated land, woodland and construction land types. (3) The impact of human activities on land use conflicts was gradually increasing. From 2010 to 2020, the basic ratio of land use conflict increased from 0.197 to 0.487, and the spatial correlation weakened, and the influence of natural factors on land use conflict gradually weakened. From 2010 to 2020, land use conflicts in Chengdu-Chongqing urban agglomeration intensified, and the impact of human activities on conflicts was strengthened. Land supervision and legal protection measures should be strengthened in the subsequent development.