Abstract: Water supply affects the diversity and behavior of animals, but the influence of water sources on animals is rarely considered in urban green space planning. In order to study the influence of water sources on animal diversity and activities in urban green spaces, from February 2018 to August 2019, we used 4 camera traps near ponds and other water sources and 3 trigger infrared cameras in areas without water sources in Dali University, to monitor and analyze the differences of species diversity, activity rhythm between rainy and dry seasons and activity rhythm of different species. The results were as follows: (1) A total of 58 species of birds and 6 species of mammals were recorded in this survey, and the capture rate and bird species diversity in water source areas were higher than those in non-water source areas. The mammal diversity in water source ares and non-water resourc ares of non-construciton area was higher than that in construction area. (2) The six species of birds and mammals with relatively high abundance were Tupaia belangeri, Callosciurus erythraeus, Pycnonotus xanthorrhous, Pycnonotus aurigaster, Garrulax sannio, Turdus dissimilis. The nuclear density estimation analysis showed that the peak activity of all species in dry season was earlier than that in rainy season. Estimation analysis revealed that all species’ peak activity occurred earlier in the dry season than in the rainy season, with no significant differences in Tupaia belangeri’s daily activity rhythm in rainy saeson and dry season, but significant differences in the daily activity rhythm of other animal groups in rainy season and dry season. (3) There was no significant differences in daily activity rhythm among Tupaia belangeri, Callosciurus erythraeus and Garrulax sannio, but the daily activity rhythm of other species overlapped to a moderate or higher degree, but the daily activity rhythm was significantly different. The daily activity rhythms of human beings and six kinds of birds and mammals overlapped to a low degree, and the activity rhythms were significantly different. There was a low degree of daily activity rhythms overlap between humans and six birds and mammal species, and there was a significant difference in activity rhythm. The study proved that the utilization of water resources by animals was affected by demand and interference from other species and humans. It suggests that the use of water resources by animals should be considered in the design and management of green space such as campus in the future.