Abstract: Cinnamomum longepaniculatum is a second-class key protected plant in China, and it is also an economic forest rich in natural aromatic oil, which is unique to China. Currently, there were few studies on Cinnamomum longepaniculatum seeds, and there was no in-depth study on how the germination rate and physiological index of Cinnamomum longepaniculatum seeds change after gibberellin treatment. In order to promote the rapid germination of Cinnamomum longepaniculatum seeds, the plant growth regulator GAs was used to treat them. Through the determination of various physiological index and the observation of morphological structure, the theoretical basis of Cinnamomum longepaniculatum seeds germination could be further enriched. Taking Cinnamomum longepaniculatum seeds as the experimental materials, four concentrations of GAs (0.2 g·L⁻¹, 0.4 g·L⁻¹,0.6 g·L⁻¹,1 g·L⁻¹) treat the seeds for 24 h and 48 h, the physiological indexes and embryo development were compared. The results showed that 0.4 g·L⁻¹ gibberellin treatment could significantly increase SOD and CAT activities, significantly decreased MDA content and significantly increased soluble protein content in Cinnamomum longepaniculatum seeds. The morphological development of embryos was obviously better than other treatments, and the endosperm cell connection density was smaller, the lipid droplets content was higher and the cell wall was thinner. Therefore, soaking seeds with 0.4 g·L⁻¹GAs for 48 h was beneficial to promote the germination of Cinnamomum longepaniculatum seeds.