Abstract: In this experiment, Sichuan local lacquer tree root sections were used as the material, and the effects of the tree age, root diameter, concentration of rooting agent and treatment time on the rooting rate of lacquer were discussed. Through comprehensive analysis, the most suitable combination of lacquer root propagation seedlings was selected. The results showed that rooting agent, tree age and rooting agent concentration had significant effects on the survival rate of lacquer tree root propagation, while soaking time and root diameter had no significant effect on the survival rate. Among the interaction between rooting agent and various factors, rooting agent × soaking time, rooting agent × tree age had very extremely significant effects on the survival rate, rooting agent × root diameter had significant effect on the survival rate, and rooting agent ×rooting agent concentration had no significant effect on the survival rate. The most suitable combination of factors selected in this study for lacquer tree root breeding seedlings in southern Sichuan was: rooting agent GGR, tree age of one year, root diameter of 0.3–0.5 cm, rooting agent concentration of 100 mg·L⁻¹ and soaking time of 10 min.