Abstract: The plant health is closely related to phyllosphere fungi, and their community structure and diversity are determined by the mutual activities of plant itself, the microorganisms and other environmental factors. In order to understand the differences of the community structure and diversity of the phyllosphere fungi which comes from different plants in the same habitat, and to determine the influences of different plants on the composition of phyllosphere fungi. The next generation sequencing method were applied to investigate the phyllosphere fungi community structures from three garden plants, including Ficus altissima, Magnolia grandiflora, Eriobotyra japonica, which were located within 50 meters in distance and around the campus of Dali University. The results showed that the alpha diversity of phyllosphere fungi was ranked as M. grandiflora > E. japonica > F. altissima. For the OTU abundance, the genus Cladosporium from phylum Ascomycota was the dominant taxon in all of the three plant phyllosphere fungi communities but the relative abundance was different. In addition, fungus communities from different plants had various endemic taxa, and the assemblies were distinct. The community structures and diversity of plant phyllosphere fungi were determined by the plant species, and the characteristics of different plants could shape their unique phyllosphere fungus communities.