Abstract: Using the branches of Lacquer as materials,, the influence of tree age, substrate ratio, rooting agent concentration and treatment time on the rooting rate of lacquer tree cuttings were analyzed by orthogonal experimental design, and the most suitable lacquer tree cutting system was comprehensively selected. The results showed that when three different rooting agents were used, the effect of branch age on rooting rate was extremely significant (P <0.01), and the younger the tree age, the higher the rooting rate. The effects of substrate, concentration and soaking time on rooting rate of Lacquer cuttings treated with GGR and IBT were not significant, but they were significant when treated with ABT (P<0.05). Comprehensive analysis of 27 treatments showed that ABT was the most suitable rooting agent for lacquer tree cuttings. When the ABT was used, the rooting rate of cutting with 1-year-old lacquer tree seedlings as cuttings, yellow sand as substrate, concentration of 500 mg·L⁻¹ and soaking for 30 min was the highest.