Abstract: In order to analyze the effects of different layers of soil on the growth of Cunninghamia lanceolata seedlings, the container seedlings of the third generation seed orchard of C. lanceolata were taken as the research object, and different layers of soil from three C. lanceolata stands were used for pot experiment, namely, layer A （0−20 cm）, layer B （20−40 cm） and layer C （40−60 cm）.After one year, the growth of C. lanceolata was measured, and the correlation between soil organic carbon, total nitrogen and C/N ratio and the growth of C. lanceolata seedlings was analyzed, The results showed that: （1） The organic carbon content, total nitrogen content and C/N ratio of layer A soil （0−20 cm） were 19.4 g·kg⁻¹、1.2 g·kg⁻¹ and 15.5 respectively. The seedling growth performance of was the best, and the seedling height and ground diameter were 85.9 cm and 16.8 mm respectively. （2） The organic carbon content, total nitrogen content and C/N ratio of layer B soil （20−40cm） were 16.7 g·kg⁻¹, 1.1 g·kg⁻¹ and 15.3 respectively. The seedling height and ground diameter were 75.6 cm and 14.5 mm respectively. （3） The organic carbon content, total nitrogen content and C/N ratio in layer C soil （40−60cm） were 3.8 g·kg⁻¹、0.5 g·kg⁻¹ and 7.8 respectively. The growth performance of seedlings was poor, and the seedling height and ground diameter were 75.0 cm and 13.4 mm respectively. Through correlation analysis, the growth of C. lanceolata was positively correlated with organic carbon content, total nitrogen content and C/N ratio.