Abstract: In order to select appropriate substrate ratio of Hippophae rhamnoides L. in the front mountain belt of the northern slope of Tianshan mountains, garden soil, decomposed sheep droppings and river sand were used as the experimental material. The physiological growth index of Hippophae rhamnoides seedling with different ratios of seedling substrates were compared and analyzed by means of variance analysis, principal component analysis, stepwise regression analysis, etc. Finally, a comprehensive evaluation of Hippophae rhamnoides L. container seedlings was made to reveal the growth characteristics of seedlings in this area, and to selected a better ratio of seedling substrates. The results showed that there were differences in the growth and physiological index of Hippophae rhamnoides L. in different seedling substrates. The optimum substrate ratio of Hippophae rhamnoides was: garden soil: decomposed sheep droppings: river sand =6:2:2.