Abstract: Ginkgo biloba L. is an excellent ornamental tree species with timber, fruit and medicinal values. The research on cutting technology of Ginkgo is of great significance to the development and utilization of Ginkgo resources. In this study, the cutting technology of Ginkgo 'Pixian No.2' was studied. The results showed that the best cutting effect was achieved by using middle branches cutting, cutting thickness 0.5 cm—1.1 cm, cutting length 14 cm—16 cm, cutting depth 11 cm, and NAA concentration 100 mg·L⁻¹ for soaking for 20 h and the survival rate could reach 85.6%. Through the analysis of Ginkgo hardwood cutting seedling related factors on the survival rate of cutting, the hardwood cutting system of Ginkgo 'Pixian No.2' suitable for Henan Province was established, which improved the survival rate of cutting, reduced the production cost, and provided scientific basis for the expansion and utilization of Ginkgo resources.