Abstract: The excellent varieties of Liriodendron sino-americanum were selected as the study object, the cuttings of the current year in the cutting orchard were used as the cuttage, and the effects of cutting season, substrate and plant growth regulators on rooting of Liriodendron sino-americanum were studied. The results showed that: (1) The rooting rate of cuttage varied significantly in different seasons, with the highest rooting rate being 57.3% in summer, followed by autumn (40.3%) and spring (24.7%). (2) There were significant differences in rooting rates among different cutting media. The rooting rate of perlite+vermiculite+river sand (1∶1∶1) was the highest (65.1%), followed by vermiculite+river sand (1∶1) (59.6%), perlite+river sand (1∶1) (52.3%), river sand (51.9%), peat soil (40.3%) and loess (33.5%). (3) Five kinds of plant growth regulators, such as ABT1, GGR7, 911, NAA and IBA, had very significant effects on cutting rooting rate. Based on the overall effect of plant growth regulators, ABT1 > NAA>GGR7 > IBA>911, and the effect of treatment time was 1h>3h>10s>6 h. The highest rooting rate was 88.8% when treated with ABT1 1000 mg·L⁻¹ for 1 h.