川产黄精组织培养快繁技术研究

姜丽琼; 李文俊; 肖前刚; 陈文灵; 徐志萍; 浣杰; 杨洪燕; 杨敏; 宋若楠

doi:10.12172/202009020001

川产黄精组织培养快繁技术研究

Study on Tissue Culture and Rapid Propagation Technology of Polygonatum sibiricum from Sichuan Province

摘要

摘要: 以四川本土黄精优良单株的块茎为外植体，探索外植体预处理方式，最佳诱导、增殖、生根培养方案，分析并筛选出黄精最佳组织培养技术，建立四川黄精组培技术体系。研究结果表明：外植体最佳预处理方式为75%乙醇溶液表面消毒1 min，然后用0.1% HgCl₂溶液浸泡15 min；最佳诱导培养基MS+ 6-BA5.0 mg·L⁻¹+ NAA0.1 mg·L⁻¹；最佳增殖培养基MS+6-BA0.5 mg·L⁻¹+NAA0.05 mg·L⁻¹+1/3(Mg²⁺+Fe³⁺)↑ +1/2KH₂PO₄↑+ AC 1 g·L⁻¹；最佳生根培养基MS +6-BA0.05 mg·L⁻¹+NAA1.0 mg·L⁻¹+ AC 0.5 g·L⁻¹，生根率可达到85%以上，室外炼苗移栽，生根幼苗和未生根的幼苗存活率达95%以上。

Abstract: The tubers of Polygonatum sibiricum were selected as explants, and the pretreatment method of explants, the best induction, proliferation and rooting culture scheme were explored. The best tissue culture technology of Polygonatum sibiricum was analyzed and screened, and the tissue culture technology system of Polygonatum sibiricum was established. The results showed that the best pretreatment method of explants was to disinfect the surface of explants with 75% ethanol solution for 1 min, and then soak in 0.1% HgCl₂ solution for 15 min. The best induction medium was MS + 6-BA 5.0 mg·L⁻¹ + NAA 0.1 mg·L⁻¹. The best proliferation medium was MS + 6-BA 0.5 mg·L⁻¹ + NAA 0.05 mg·L⁻¹ + 1/3 (Mg^{2 +} + Fe^{3 +}) ↑+ 1/2 KH₂PO₄↑+ AC 1 g·L⁻¹. The best rooting medium was MS + 6-BA 0.05 mg·L⁻¹ + NAA 1.0 mg·L⁻¹ + AC 1 g·L⁻¹ 5 g·L⁻¹, and the rooting rate could reach more than 85%. The survival rate of rooting seedlings and non-rooting seedlings could reach more than 95% after outdoor acclimatization and transplanting.

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川产黄精组织培养快繁技术研究

Study on Tissue Culture and Rapid Propagation Technology of Polygonatum sibiricum from Sichuan Province

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