Abstract: In order to develop an efficient approach for the rapid propagation of Oriental hybrid lily cultivar ‘Vivian’ by tissue culture, the scales were used as the explants. The results showed that the best adventitious bud induction medium of Oriental hybrid lily cultivar ‘Vivian’ was MS+1.0 mg·L⁻¹ 6-BA+0.1 mg·L⁻¹ NAA+30 g·L⁻¹ sucrose+6.0 g·L⁻¹ agar, and pH value was 6.0. The optimal medium for adventitious bud proliferation was MS+1.0 mg·L⁻¹ 6-BA+0.15 mg·L⁻¹ NAA+60 g·L⁻¹ sucrose+6.0 g·L⁻¹ agar, and the pH value was 6.0. The best medium for bulblet expansion was MS+ 90 g·L⁻¹ sucrose+6.0 g·L⁻¹ agar, with pH value of 6.0. The optimal medium for bulblet rooting was 1/2 MS+0.3 mg·L⁻¹ NAA+60 g·L⁻¹ sucrose+6.0 g·L⁻¹ agar, and the pH value was 6.0. Transplanted tissue culture seedlings in the substrate of the ratio of peat to vermiculite of 1∶1 growed vigorously and the survival rate was up to 95.31%. This efficient in vitro rapid propagation system can provide technical support for large scale production of Oriental hybrid lily cultivar ‘Vivian’.