Abstract: In order to study the effects of different substrates ratios on the growth, biomass and seedling quality of Phoebe zhennan seedlings, the half-year-old Phoebe zhennan seedlings were used as the test materials with the L16 (5⁴) orthogonal experiment. The results showed that different substrates ratios had significant effects on the seedling height, ground diameter, leaf dry weight, branch dry weight and root dry weight of 1- and 2-year-old Phoebe zhennan seedlings (P<0.05). The substrate ratio of coconut bran (4), peat soil (6), oil camphor leaf (2), agricultural and forestry waste (1), humus soil (4) was the best treatment with the best growth indexes and balanced performance. And the comprehensive evaluation results were the best, among wich the ground diameter and seedling height in the second year increased by 5.18 mm and 50.23 cm respectively compared with the first year. The second best substrate ratio was coconut bran (6), peat soil (6), oil camphor leaf (1), agricultural and forestry waste (3), humus soil (2), in which the ground diameter and seedling height in the second year increased by 4.06 mm and 45.99 cm respectively compared with the first year. Effect of different substrate components on the growth of Phoebe zhennan seedlings were as follows: peat soil > agricultural and forestry waste > humic soil > oil camphor leaf > coconut bran,