Abstract: In order to study the effect of different substrates on the growth and development of Haloxylon ammodendron under different seedling growth conditions, using H. ammodendron as the studying materials, the effects of three different substrate configurations and two seedling growth conditions on the growth conditions of H. ammodendron were studied. The results showed that: (1) under the conditions of small arch shed nursery and open-air nursery, the best nursery substrates were both garden soil: river sand: decomposed sheep manure = 1:1:1. (2) under the same substrate, the seedling growth in the small arch shed nursery methods was better than that in open-air nursery. (3) when sowing, the seedling growth rate was the best when using the small arch shed nutrient bag nursery method and the nursery substrate was garden soil: river sand: decomposed sheep dung = 1:1:1. The average seedling height, average ground diameter, average main root length, first-class side root length, and seedling weight was 39.28 cm, 0.19 cm, 11.83 cm, 7.45 cm, and 6.87 g respectively.