Abstract: In order to screen high-quality mulberry varieties and explore the differences of anthocyanin and soluble sugar content in different mulberry varieties, Langchang No.1, Langchang No.2, Langchang No.3, Langchang No.4, Langchang No.5, Langchang No.6 and Langchang No.7 were selected as the experimental materials in Langzhong silkworm breeding farm. High performance liquid chromatography method and general analysis method were used to detect plant-derived anthocyanin and soluble sugar content respectively in different mulberry varieties. The results showed that Langchang No.4 was the best mulberry variety with the highest anthocyanin and soluble sugar content, reaching 1.024 mg·g⁻¹ and 16.348 mg·g⁻¹ respectively. Therefore, Langchang No.4 was the best fruit mulberry variety suitable for popularization.