Abstract: Eupatorium adenophorum (Ageratina adenophora) is widely distributed in China. In order to explore new methods of resource utilization of Eupatorium adenophorum, the powder of roots, stems and leaves of Eupatorium adenophorum was used as the additive of the culture medium. By detecting the changes of the number and speed of microorganism propagation in the culture medium, we explored whether the powder of Eupatorium adenophorum could promote the propagation of Bacillus subtilis in the culture medium. The results showed that the dry powder of the roots, stems and leaves of Eupatorium adenophorum had a certain promotion effect on the growth of Bacillus subtilis in the culture medium, and the dry powder could more effectively improve the propagation speed of Bacillus subtilis in LB liquid culture medium.