Abstract: Pomacea canaliculata is rich in protein and high in nutritional content. If the ability to efficiently transform fiber into animal protein can be applied to bamboo resources, it is of important practical significance to increase bamboo utilization ways and promote the development of bamboo industry. In this study, the regression orthogonal design was used to determine the effect of adding bamboo (Neosinocalamus affinis) fiber content in bait on the growth rate of P. canaliculata. The results showed that when the bamboo fiber content in the bait reached 50%, the growth rate of P. canaliculata increased significantly, and its weight growth rate could reach 85.5%. The growth rate of P. canaliculata was mainly composed of three treatment effects: N. affinis fiber, soybean meal and corn flour. The addition of N. affinis fiber to bait had the largest contribution to the weight gain rate of P. canaliculata, and reached the significant level (p<0.01). The regression equation for the weight rate Y and N. affinis fiber X₁, soybean meal X₂, and corn flour X₃ was Y=13.862X₁+8.988X₂+5.237X₃+ 55.358. The results of this study provided a theoretical basis for the transformation of bamboo fiber into animal protein, and had important reference values for the feed utilization of bamboo fiber.