Abstract: From April to October, the semi-lignified current-year branches with axillary buds of Sarcandra glabra were cut every month as explants to carry out tissue culture experiments. The results showed that (1) the best ideal explants of Sarcandra glabra was the base stem segment of semi-lignified branches from April to May. (2) The best incubation medium for explants was B5 + 0.8 mg·L⁻¹ 6-BA + 0.3mg·L⁻¹ NAA. The best proliferation medium was B5 + 3.0mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ NAA, with an average proliferation coefficient of 6.82. The best rooting medium was 1/2B5 + 0.8 mg·L⁻¹ IBA + 0.1mg·L⁻¹ NAA, the rooting rate was 100%, and the average number of roots was 6.51. (3) With humus soil: coconut husk: perlite: soil = 5:2:1:2 as the transplant substrate, the transplant average survival rate was 94%, the tissue culture seedlings grew well, with thick green leaves and strong plants.