Abstract: Urban ecosystem service is the research hotspot of ecological economics and environmental economics. Based on the literature records of urban ecosystem services research in CNKI from 1999 to 2019, the research hotspots and trends on urban ecosystem services in China were explored by using knowledge map analysis and bibliometric method. The results showed that: (1) The domestic research showed a continuous growth trend, which could be divided into three stages: preliminary exploration, steady growth, and rapid growth. (2) The research hotspots had gradually shifted from urban ecosystem service functions and value assessment to urban ecological planning and ecological restoration research based on ecosystem services. (3) Further research hotspots include: spatial evaluation and mapping of urban ecosystem services, evaluation index and methods of urban ecosystem service value, trade-off and coordination of urban system services, relationship between urban system services and well-being of urban residents, and application of urban system services in urban planning and design.