Abstract: The effects of different planting matrixes were studied on the growth of Dendrobium officinale in the Panxi dry-hot valleys.The results showed that the roots of D. officinale grew rapidly in the first and third quarter of the year, and the buds sprouted mainly in the first and fourth season, the rapid growth of fresh weight happened in the second and third quarter of the year. There were significant correlations among growth traits such as plant stem diameter and height. For planting D. officinale in greenhouse in Panxi dry-hot valley areas, the most suitable matrix was the recipe D, which was made of saw powder, pine bark and shavings in a volume ratio of 1:3:1.