Abstract: The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+ IBA 0.1 mg·L^-1; The medium for multiplication of adventitious buds was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+NAA 0.10 mg·L^-1+IBA 0.2 mg·L^-1; The rooting medium was sucrose 15 g·L^-1+carrageenan 6 g·L^-1+1/2MS+IBA 1.5 mg·L^-1+NAA 1.0 mg·L^-1.