四川紫花白及无菌播种技术研究

Research on the Aseptic Seeding Technique of Bletilla striata with Lilac Flowers

摘要: 以当年成熟白及果荚种子作为外植体,开展组织培养试验研究。结果表明:最佳外植体诱导培养基为改良MS+0.2 mg·L^-16-BA+0.2 mg·L^-1NAA,发芽率可达90%~100%;最佳增殖培养基为改良MS+2.0 mg·L^-16-BA+0.3 mg·L^-1NAA,增殖系数3.4;最佳生根培养基为改良MS+0.2 mg·L^-1 NAA+1 mg·L^-1 AC+50 g·L^-1香蕉泥培养基,生根率为95.6%。炼苗基质为腐殖土:珍珠岩:椰糠:河沙=5:1:2:2组合,为白芨移栽最佳基质,移栽苗存活率可达97%。

Abstract: Taking the ripe seeds that removed from the inflated pods as explant,experiments were conducted on this tissue culture.The results showed that the best inductive medium for explant culture was an improved fromula listed as follows:MS+0.2 mg·L^-16-BA+0.2 mg·L^-1NAA,and the germination rate could be generally up to 90%~100%;The best enriched medium was an improved formula listed as follows:MS+2.0 mg·L^-16-BA+0.3 mg·L^-1NAA,and the propagation coefficient was 3.4;The best rooting medium was an improved formula of banana puree substrate listed as follows:MS+0.2 mg·L^-1NAA+1 mg·L^-1AC+50 g·L^-1,and the rate of rooting was 95.6%.The growth substrate was a combination of humus:perlite:coconut hust:fluvial sand=5:1:2:2,which was the best transplanting substrate for Bletilla striata,and the survival rate of the transplanting reached 97%.