Abstract: The Larch mature zygotic embryos were taken as the explants. They were disinfected according to different treatment methods,and induced by use of different medium and hormone types and concentration,then the callus volume were calculated. Statistical analysis results showed that the peeled seeds were washed with water for 24 hours~48 hours,disinfected in 70% alcohol for 1 minute and disinfected into 4% NaClO for 10 minutes,and then the embryo was stripped out in the 0.5% NaClO disinfection for 2 minutes and the suvival rate of embryos was the highest; In the light conditions,MS medium was good medium for callus induction. It could be seen that 2.0 mg·L^-1 2,4-D + 1.0 mg·L^-1 6-BA or 1.0 mg·L^-1 NAA + 0.5 mg·L^-1 6-BA were the best hormone combination.