Abstract: In different matrix of Alnus nepalensis cutting test,use was made of a completely randomized block design. For cutting Alnus nepalensis in three matrix (forest soil, river sand and perlite) in Research Institute of Tropical Forestry of Yunnan Academy of Forestry(Puwen), the most suitable was forest soil. The average rooting rate was 43.57%, the average number of roots was 6.06, the average branching length was 4.26 cm,and the average root length was 9.39 cm.