Abstract: In order to screen the optimal transplanting substratum, 8 kinds of substrata or mixed substrata were used for transplanting test-tube plantlets of Cymbidium hybridium,and sphagna were used as a control. Results indicated that rape straw (20 mm~30 mm particle size) would be the optimum substratum for transplanting the test-tube plantlets of Cymbidium hybridium in Chengdu Plain, because it would bring a higher survival rate and growth index of plantlets, and had an abundant supply and lower costs.