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山茶花粉生活力及贮藏条件初探

A Study of the Viability and Storage Conditions of Camellia Pollen

  • 摘要: 以山茶两种开放程度花朵(初开和盛开)的花粉为试材,采用无机酸法和联苯胺染色法研究了花朵开放程度和贮藏条件对花粉生活力的影响。结果表明:无机酸法不适于山茶花粉生活力的测定,联苯胺染色法能快速地检测山茶花粉的生活力。实验结果表明,两种花粉生活力随着贮藏时间的增加呈逐渐下降的趋势。花朵的开放程度对花粉的生活力影响不大,而贮藏条件对其影响显著:室温(13℃~25℃)下,两种山茶花粉贮藏12 d后生活力完全丧失;冷藏(2℃)条件下,盛开花粉贮藏26 d后生活力完全丧失,而初开花粉生活力完全丧失需要30 d。

     

    Abstract: The Camellia pollen (in the early opening and in full bloom) was used as test materials. The effects of flower opening degree and storage conditions on the pollen viability were studied by means of benzidine staining and inorganic acid. The results showed that the inorganic acid method was not suitable for the determination of the pollen viability of Camellia,but the benzidine staining method could determine quickly the pollen viability of Camellia. The viability of the two kinds of pollen was high on average,and yet gradually declined along with the prolonging of the preserving period. The degree of flower opening did not effect obviously on the pollen viability,but the effects of storage conditions were significant. The two kinds of pollen could be stored for 12 days at the room temperature(13℃~25℃) without loss of viability. Under 2℃ cold storage condition, the pollen in full bloom could be stored only for 26 days without loss of viability while the pollen in early opening only for 30 days.

     

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