Abstract: Forestry carbon sequestration has become a very important method in reducing greenhouse gases emission, and the estimation method of forest carbon sequestration is the basic content for our country in formulating strategy on how to deal with climate changes, performing the international convention, regularly working out and reporting greenhouse gas inventory. Therefore, it is urgent to carry out researches on the estimation method of forest carbon sequestration. From the perspective of estimation method of forest carbon sequestration, this paper analyzes the coupling of forest resource investigation and the estimation method of forest carbon sequestration in our country, and indicates that it is possible to apply forest resource investigation data into the estimation method of forest carbon sequestration. This paper also points out that the application of forest resource investigation data into the estimation method of forest carbon sequestration has such advantages as timely convenience and data reliability. Meanwhile, such preliminary information supplemented in forest resource investigation as soil organic carbon, forest management, varieties and hierarchy of trees, bushes and grass seeds, dead standing trees and fallen trees will meet the demands of estimating and monitoring of forest carbon sequestration.