Abstract: Quercus fabri, as an important native tree species in China, holds significant value for ecological restoration and timber forest construction. However, a standardized cultivation system for Q. fabri container seedlings has not yet been established, particularly lacking systematic research on the synergistic optimization of substrate formulation, container specification selection, and fertilization regimes. This study aims to develop an efficient cultivation system for Q. fabri container seedlings through multifactorial controlled experiments, providing high-quality nursery stocks for plantation establishment.The experiment was conducted from 2023 to 2025 using Q. fabri seedlings. An L₉(3⁴) orthogonal design was employed to evaluate three key factors: (1) substrate ratio (Factor A: coir:peat:soil volume ratios of 2:1:1, 1:2:1, and 1:1:2), (2) container size (Factor B: non-woven fabric bags of 8×12 cm, 10×15 cm, and 12×16 cm), and (3) slow-release fertilizer dosage (Factor C: 0, 2, and 4 kg·m⁻³). For one-year-old seedlings, growth was significantly influenced by fertilizer dosage, but not by substrate ratio or container size. All three factors exhibited significant effects on two-year-old seedlings. Biomass varied markedly among treatments, showing correlation with seedling height than other parameters. The optimal combination (A2B2C3) demonstrated a mean membership function value of 0.95, producing two-year-old seedlings with 129.01 cm height and 1.06 cm ground diameter while balancing growth performance and economic cost.The recommended protocol for cultivating premium two-year-old Q. fabri container seedlings (height >1.2 m, diameter >1.0 cm) comprises: 1) 1:2:1 coir:peat:soil substrate, 2) 4 kg·m⁻³ slow-release fertilizer, and 3) 10×15 cm non-woven containers. This system provides a technical reference for standardized production of native broad-leaf container seedlings.