Abstract: To establish a suitable cutting technology system for Ormosia hosiei in Sichuan, this study conducted a cutting experiment with Sichuan-sourced O. hosiei in Yuchan Mountain, Luxian County, Luzhou City, Sichuan Province. The changes of soluble sugar contents and oxidase (PPO, POD, NAAO) activities during rooting were analyzed. The variance experimental design was used to analyze the effects of meduim type, growth regulator type, growth regulator concentration, and growth regulator treatment time on cuttage rooting of O. hosiei. The results showed that during the rooting process of cuttings in both the growth regulator treatment group and the control group, the soluble sugar content, PPO and POD activities initially increased and subsequently decreased, while NAAO activities showed an opposite trend. The inflection point for these changes occurred earlier in the growth regulator treatment group compared to the control group. The growth regulator type, medium type, growth regulator concentration, the interaction between growth regulator and concentration, as well as the interaction between growth regulator and treatment time, all had extremely significant effects on the rooting rate of O. hosiei. The highest rooting rate and average length of new shoots were observed in the pure vermiculite medium, while the largest average number of thick roots was found in the medium with a volume ratio of 75% vermiculite and 25% perlite. Both ABT and NAA resulted in relatively high average rooting rates, with ABT showed greater average lengths of new shoots and fresh weight compared to NAA. Among the ABT treatment combinations, higher concentrations (800 mg/L) with shorter treatment times (10 min, 30 min) yielded higher average rooting rates. This research indicated that the application of growth regulators could accelerate the dynamic changes of soluble sugar content and oxidase activities in cuttings, thus enhancing root formation. Through mean comparison analysis, the optimal combination of factors for O. hosiei cuttings is determined to be the use of vermiculite as the medium coupled with an 800 mg/L ABT treatment for 10 to 30 minutes.