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普通油茶IQD基因家族鉴定及果形候选基因挖掘

Identification of IQD gene family and exploration of candidate genes regulating fruit shape in Camellia oleifera

  • 摘要: IQD基因家族编码一类植物特有的钙调素结合蛋白,其部分家族成员对果实形状具有重要影响,对该家族的研究有助于油茶(Camellia oleifera)的果形改善与产量提高。基于基因组、转录组及单细胞转录组数据,对油茶IQD基因进行鉴定及蛋白理化性质与系统发育分析,并对基因在不同组织及成熟子房不同细胞亚群的表达情况进行分析。油茶基因组中含有48个CoIQD基因;系统进化树分析表明,CoIQD可以分为4个亚家族、9个分支,分布在I-1及II-3分支的CoIQD参与油茶果形调控的可能性最高,II-1与IV-2分支次之。基因表达分析表明,I-1分支CoIQD45/-47、II-3分支的CoIQD7/-14、II-1分支的CoIQD27等5个基因在成熟子房及果皮均有表达,是油茶的果形候选基因,CoIQD7/-14/-47等3个基因可能是参与油茶子房高度调控的关键候选基因。

     

    Abstract: Some members of the plant-specific IQ67 domain (IQD) gene family coding a class of calmodulin-binding proteins play vital roles in regulating fruit shape. Investigation of IQD genes will help to improve fruit shape and promote yield in Camellia oleifera. Based on the genome data of C. oleifera, the IQD genes were identified, and the physicochemical properties and phylogenetic analysis of IQD proteins were analyzed. The expression of IQD genes in different tissues and in different cell clusters of mature ovaries in C. oleifera was analyzed using bulk transcriptome and single-cell RNA-seq data. A total of 48 CoIQD genes were identified, and the 48 CoIQD proteins were divided into 4 subfamilies and 9 groups according to the topology of phylogenetic tree. The CoIQD proteins distributed in groups I-1 and II-3 were more possible than ones in groups II-1 and IV-2 as candidate regulators of fruit shape. The results of expression profiling indicated that such 5 genes as CoIQD45/-47 of group I-1, CoIQD7/-14 of group II-3, and CoIQD27 of group II-1 expressed in both mature ovary and pericarp, and were candidate genes to regulate fruit shape. CoIQD7/-14/-47 were key candidate genes involved in regulating ovary height in C. oleifera.

     

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