Abstract:
In order to replace
Lonicera japonica bud as feed additive, reduce feed cost and make full use of
Lonicera similis leaves resources, the antibacterial and antioxidant activities of crude extract, optimized extract and refined extract of
L. similis leaves were studied. The main active components of different extracts of
L. similis leaves were determined, and in vitro antibacterial experiments of each extract against
Escherichia coli,
Staphylococcus aureus,
Salmonella typhimurium and
Pseudomonas aeruginosa were carried out, and the MIC and MBC experiments were carried out on the refined products with better antibacterial effect. The total antioxidant capacity of each extract was determined by ABTA, DPPH and FRAP. The main results are as follows: (1) The refined products of
L. similis leaves showed significant antibacterial activity in the antibacterial activity experiment, and the antibacterial ability was similar to that of the flower buds of
Lonicera japonica, and higher than that of the crude extract and optimized products. (2) The MIC of
L. similis leaves refined products against
Escherichia coli,
Staphylococcus aureus,
Salmonella typhimurium and
Pseudomonas aeruginosa were 12.5 mg·mL
−1, 6.25 mg·mL
−1, 1.56 mg·mL
−1 and 3.125 mg·mL
−1, respectively, and the MBC were 25 mg·mL
−1, 12.5 mg·mL
−1, 3.125 mg·mL
−1 and 12.5 mg·mL
−1 respectively. (3) The
L. similis leaves refined products exhibited strong antioxidant activity, and its antioxidant capacity gradually increased with the increase of solution concentration, and the antioxidant capacity was higher than that of crude extract, optimized product and refined
L. japonica extract, indicating that the antioxidant capacity of
L. similis Hemsl. after enrichment and purification was significantly increased. The refined product of
L. similis leaves had good antibacterial and antioxidant activities, and had the potential to replace
L. japonica as feed additive.
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