Abstract: The composition and diversity of moth in spatial differences were compared through collecting the specimen of moth (Lepidoptera) using light trap from July to October 2020 in three habitats, including deciduous broad-leaved forest, coniferous and broad-leaved mixed forest, and alpine coniferous forest and meadow, in Jiuzhaigou National Nature Reserve in Sichuan Province. A total of 1430 adult moths belonging to 16 families, 151 genera, and 227 species were collected, among which Noctuidae had the highest number of species and Geometridae had the highest number of individuals. The diversity results showed that the Margalef richness index and Shannon diversity index of moth in deciduous broad-leaved forest, coniferous and broad-leaved mixed forest plots were higher than those in alpine coniferous forest and meadow plot, and the community performance was stable. Among them, the Margalef richness index showed obvious differences in the diversity characteristics of family, genus, and species, while the changes of other indexes were consistent. The results of community similarity analysis showed that the similarity of moths collected from deciduous broad-leaved forest and coniferous and broad-leaved mixed forest was the highest (0.4178), moth community in the alpine coniferous forest and meadow had a low similarity with those in the other two habitats (P<0.1).