Abstract: Taking the leaves of 13 introduced varieties of Saintpaulia species as the explants, the sterilization plan and the induction, the proliferation, the rooting culture plan of Saintpaulia explants were explored, and the leaf test tube rapid propagation system of Saintpaulia was preliminary established. The results showed that the acquisition rate of sterile explants treated by 0.1% HgCl₂ for 5 minutes and 10% 84 for 8 minutes was 67.9% and 72.8%, respectively. Medium for callus induction: MS + sucrose 30 g·L⁻¹+ carrageenan 6.5 g·L⁻¹+6-BA 0.15 mg·L⁻¹+NAA 0.1 mg·L^-1. Better formula for proliferation: MS + sucrose 30 g·L⁻¹ + carrageenan 6.5 g·L⁻¹ + 6-BA 0.2 mg·L⁻¹ + NAA 0.1 mg·L⁻¹ + IBA 0.15 mg·L⁻¹, and the average effective proliferation coefficient could reach 6.43. The optimal rooting formula: 1/2MS + sucrose 15 g·L⁻¹ + carrageenan 6.0 g·L⁻¹ + IBA 1.0 mg·L⁻¹ + NAA 0.05 mg·L⁻¹, and the average rooting rate was 92.67%.