Abstract: From July to August 2015, an investigation of Staphylinidae species and the host macrofungi was conducted in Wolong nature reserve. A total of 359 Staphylinidae specimen were collected, which were identified as belonging to 6 subfamilies, 17 genera and 25 species. There were 2 dominant subfamilies: Aleocharinae, 6 genera and 9 morphological species, accounting for 56.2% of the total specimen; Tachyporini, 4 genera and 6 morphological species, accounting for 29.8% of the total specimen. A total of 105 samples of macrofungi belonging to 10 families, 14 genera, 26 species were collected, including 16 edible macrofungi, 7 poisonous fungi and 8 medicinal anticancer fungi. Tricholomataceae and Russulaceae were dominant families, accounting for 23.1% and 19.2% of the total species respectively. The analysis of α diversity of Staphylinidae species in four main host macrofungi showed that the species and quantity of Staphylinidae parasitized by Tricholomataceae were the highest, with the highest species diversity (H') and evenness (J), followed by Russulaceae and Mycenaceae. The predominance index (λ) of parasitic Paederus in Boletaceaei was the highest, while the species diversity and evenness are the lowest. In this study, the species of fungi-feeding Staphylinidae and the host macrofungi were investigated, which provided basic information for the further research of their interactions.