Abstract: The seedling substrates were prepared with the fungal residue, mushroom residue, peat, chaff, field soil and slow-release fertilizer, and the effect of substrate with different formulas on the growth of container seedlings of Torreya grandis was carried out. The results showed that: (1) Different substrates with different formulas had significant effects on seedling height, ground diameter and biomass of the container seedlings of Torreya grandis. The average quality index of Torreya grandis seedling cultivated by the compound substrate with fungal residue or with mushroom residue was better than that of peat substrate. Among them, the compound substrate formula A2 with the volume ratio of 60∶20∶20 for fungal residue, chaff and field soil was the best for the seedling weight, ground diameter and root growth. (2) The compound substrate with fungal residue greatly reduced the cost of seedling cultivation, and the cost of compound substrate with fungal residue was only 35.8% of the cost of peat compound substrate. The application of mushroom residue substrate realized the recycling utilization of mushroom residue, greatly reduced the seedling cultivation cost and improved the seedling quality of Torreya grandis.