Abstract: Using proliferating seedling of Sorbus hupehensis of more than 5 generations as explants, methods to improve the induction and growth of adventitious buds were studied. The results showed that among the 23 additives, agarose and konjac powder can significantly improve the rooting rate of sprouts, and the medium with added konjac powder has the best rooting effect. In the rooting medium of DKW+0.5 mg/L NAA containing 1 g/L konjac powder, the rooting rate of Sorbus hupehensis seedlings was significantly increased from 31.67% in the control to 52.86%. When the konjac powder concentration was increased to 2 g/L, the rooting rate increased significantly to 80.95%, and a large number of adventitious roots and lateral roots could be induced. Each sprout had an average of 5.39 adventitious roots, an average root length of 21 mm, and 6.19 lateral roots, with an average lateral root length of 6.7 mm. At the same time, the stem segments of the sprouts stretch, and the average seedling height can reach 4.3cm.