A Study of Tissue Culture of Cinnamomum longepaniculatum

The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+ IBA 0.1 mg·L^-1; The medium for multiplication of adventitious buds was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+NAA 0.10 mg·L^-1+IBA 0.2 mg·L^-1; The rooting medium was sucrose 15 g·L^-1+carrageenan 6 g·L^-1+1/2MS+IBA 1.5 mg·L^-1+NAA 1.0 mg·L^-1.