Induction of Callus and Adventitious Bud in Larix olgensis

The mature zygotic embryo of Larix olgensis was used as the research object.The callus and adventitious bud induction experiments were made to select disinfection method of explants, appropriate medium and hormone combination for L.olgensis.The optimum disinfection method was to sterilize peeled seeds with 4% NaClO for 15 min,and to disinfect the embryos with 0.5% NaClO for 2 min;BM was the optimum medium for callus induction;In BM medium supplemented with 2.0 mg·l^-1 TDZ,the callus induction reached the highest rate,89%;In BM medium combined with 1.5 mg·l^-1 TDZ,the adventitious bud induction reached the highest rate,80.0%.