Establishment and Optimization of ISSR-PCR Reaction System for Acer pentaphyllum

Acer pentaphyllum is a kind of rare and endangered plants and it has important scientific value in studying angiosperm's phylogeny,paleogeography and paleoclimate.It this study,2XFaq Master Mix was adopted and the effect of Taq polymerase,dNTPs and Mg²⁺ were considered aiming to establish and optimize the best ISSR-PCR reaction system for Acer pentaphyllum.The results showed that the most remarkable factor was primers,followed by DNA template,and 2X Master Mix had the least effect on the reaction result.The optimum reaction system (25 μL) was 1 μL of DNA template (50 ng·μL^-1),2 μL of primer and 13.5 μL of 2XTaq Master Mix.