A Study of Inducing 2n Pollen of Populus deltoid by Colchicines

In order to get the triploids of P.deltoid,the technique was researched to induce 2n pollen of P.deltoid by colchicines by two-factor randomized block design.The results indicated that(1)a certain proportion of 2n pollen grains could be gotten from the male buds induced by cochicine solution with the concentration of 0.3%,0.5% and 0.8%,under the condition of water culture for 3,4 and 5 days.(2)the proportion of 2n pollen grains could be the highest when the male buds were treated by the cochicine solution of 0.5% under the condition of water culture for 3 days,the percentage of A4(d≧50) could be up to 1.4%,the percentage of 2n pollen (d≧37.5) could be up to 23.4%.