Research on the Aseptic Seeding Technique of Bletilla striata with Lilac Flowers

Taking the ripe seeds that removed from the inflated pods as explant,experiments were conducted on this tissue culture.The results showed that the best inductive medium for explant culture was an improved fromula listed as follows:MS+0.2 mg·L^-16-BA+0.2 mg·L^-1NAA,and the germination rate could be generally up to 90%~100%;The best enriched medium was an improved formula listed as follows:MS+2.0 mg·L^-16-BA+0.3 mg·L^-1NAA,and the propagation coefficient was 3.4;The best rooting medium was an improved formula of banana puree substrate listed as follows:MS+0.2 mg·L^-1NAA+1 mg·L^-1AC+50 g·L^-1,and the rate of rooting was 95.6%.The growth substrate was a combination of humus:perlite:coconut hust:fluvial sand=5:1:2:2,which was the best transplanting substrate for Bletilla striata,and the survival rate of the transplanting reached 97%.