Optimization of Solid-liquid Biphasic Ferment Constitution of Nomuraea rileyi

Screening by substrates of basic medium, C, N, and vitamin, the formula of corn(50 g), glucose(1.5 g), casamino acids(1.5 g), ascorbic acid(0.5 mg), H₂O(50 mL) was optimal medium substrates(cultivation for 10 d) for Nomuraea rileyi, the sporulation quantity was 4.78×10¹⁰ cfu·mL^-1. Taking SMY as the liquid medium, the optimal fermentation conditions of N. rileyi were as below:1.0×10⁸ cfu·mL^-1 of inoculated concentration, 25℃, pH6, full light, 180 r·min^-1 of shaking culturing for 7 days, and 690.2 mg of mycelia. The solid fermentation provided the basic formula for producing compound powder, and shortened time of liquid fermentation could be used as secondary generation for producing N. rileyi powder of solid fermentation. The fermented product is the important biological preparation of control Orthosia songi.