Establishment of Rapid Propagation for sterile Seedlings of Anoectochilus emeiensis by Tissue Culture

Culture in Vitro and propagation were carried out with capsules of Anoectochilus emeiensis by using orthogonal design with 4 levels of 5 factors.The result showed that the best explants disinfection method was to immerse 30 s in 75% alcohol plus 0.1%HgCl₂;the best medium for primary culture was MS+10% banana juice;MS+10% banana+6-BA 2 mg·L^-1+NAA 0.5 mg·L^-1+AC 3g·L^-1 was the most suitable for bud induction and multiplication and the optimum medium for rooting and strong seedling culture was 1/2MS+10% banana+ NAA 2 mg·L^-1+6-BA 0.5 mg·L^-1+ AC 1g·L^-1.