A Study of Tissue Culture of Eucalyptus saligna

Studies were made of tissue culture and fast propagation system of Eucalyptus saligna by taking stem segments with auxiliary buds from superior plants' shooting as explants.The results showed that cutting the superior plants at the trunk base could get the best effect of promoting sprouting with germination rate of 100% and average number of 38 shoots.Late March was the best time to obtain explants and semi-lignified shoots were the best choice.The optimal medium for primary induction was MS+6-BA0.5 mg·L^-1+NAA0.05 mg·L^-1 with a germination rate of 66.67%.The optimal medium for subculture was modified MS+6-BA1.0 mg·L^-1+IBA0.02 mg·L^-1+NAA0.05 mg·L^-1 with a multiplication coefficient of 5.44.The optimal medium for rooting was modified MS+6-BA0.01 mg·L^-1 +IBA0.5 mg·L^-1 +NAA0.2 mg·L^-1 with a rooting rate of 95% and the average root number of above 9.