Comparison between Albescent and Normal Seedings of Phoebe zhennan by AFLP

Using the amplified fragment length polymorphism (AFLP) technique to study polymorphism between the genomic DNA of albescent seedlings and normal seedlings of Phoebe zhennan, it was found that bands amplified by P6 and P11 were between albescent seedlings and normal seedlings. The number of bands of albescent seedlings amplified by P6 was one less than that of normal seedlings and the size of the lacking stripe was about 230 bp. The number of bands of albescent seedlings amplified by P11 was one more than that of normal seedlings and the size of the extra stripe was around 200 bp. The differences of bands indicated that the DNA sequence of albescent seedlings was different from that of normal seedlings. It was needed to conduct sequence and comparative analysis to confirm whether the differences were caused by base mutation,deletion or insertion.