An Experimental Study of Tissue Culture of Chamelaucium uncinatum ‘Snow Flake’

As a new generation of woody cut flowers, C. uncinatum ‘Snow Flake’ has a high ornamental and economic value. Based on the study of tissue culture of ‘Snow Flake’,the aim of this experiment was to lay the foundation for Industrial production of ‘Snow Flake’. The results showed that 7 minutes were the best time to sterilize explants by using 0.1% HgCl2. MS1 + 6-BA1.0 mg·L^-1 + IBA0.2 mg·L^-1 + KT0.5 mg·L^-1 was the optimum primary culture medium, the best subcultuer medium was WPM1 + 6-BA 1.0 mg·L^-1 + IBA 0.2 mg·L^-1 + GA3 1.5 mg·L^-1,multiplication coefficient was 4.2,multiplication was 3~6. The most suitable rooting medium was 1/2MS2 + IBA0.2 mg·L^-1 + 0.1 mg·L^-1 GGR.