The Optimization of the SSR Reaction Program and the Reaction System in Researches on Quercus liaotungensis

In this paper,optimization researches were conducted on the SSR reaction program and the reaction system for the research process of Q. liaotungensis genetic diversity. The optimized reaction program was to adopt 33 cycles,which included 3 minutes of pre-denaturing at 94℃,45 seconds of denaturing at 94℃,45 seconds of annealing at 58℃,45 seconds of extension at 72℃,and a final extension step was carried out at 72℃ for 7 minutes. The optimized reaction system was as follows:30 ng DNA,2 μL10×Buffer(Mg²⁺ Plus),150 μmol·L^-1 dNTP Mixture,0.3 μmol·L^-1of each primer,1U(5 u·μL^-1) Taq polymerase,and the total reaction volume was 20 μL. The results had a certain reference value for the application of the SSR marker in the research on the genetic diversity of oak plants.